CHEM564 Lecture Notes - Lecture 11: Nitroso, Chymotrypsin, Deblocking Filter
2 May 2017
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Document Summary
Proteins & peptides consist of repeating amino acid residues linked by an amide bond. The protein is denoted as having an n-terminus and a c-terminus, based on the orientation of the individual amino acid residues. Hydrolysis of the peptide bond can occur in acidic or alkaline solutions, although peptides are generally stable between 2 < ph < 10. At ph 7, the rate of hydrolysis is extremely slow (3 x 10-9 s-1 at ph 7; about a 7 yr half life! Strong acid or alkaline conditions can be used to hydrolyze peptides far more rapidly. For example, 6m hcl at 110 c for 24 72 h will result in hydrolysis of the peptide. These conditions are also harsh enough to potentially degrade amino acid side chains (such as asn and. This approach has become less popular with the rise of mass spectrometry methods that provide more information with less material.
