BIOLE 207 Lecture Notes - Lecture 2: Deoxycytidine Triphosphate, Dna Replication, Helicase
Document Summary
The dna structure is made up of deoxyribonucleotide triphosphates and a phosphate deoxyribose backbone. The deoxyribonucleotide triphosphates are purines and pyrimidines which are deoxyguanosine triphosphate and deoxythymidine triphosphate, and deoxycytidine triphosphate, deoxyuridine triphosphate, and deoxythymidine triphosphate respectively. The nitrogenous bases are held by hydrogen bonds and the phosphate deoxyribose backbone strands are held together by covalent bonds (phosphodiester bonds) between the strands. The deoxyribose phosphate backbone runs in a 5" phosphate to a 3" oh direction and the other strand is antiparallel to this strand. Loci is the discrete location of genes on a dna strand arranged in a linear fashion and this allows for the ability to map genes in the genome. Meselson and stahl conducted an experiment to discern the mechanism of dna replication. They took n15 which is heavy and grew it in the lighter n14 sample medium. Afterwards, they extracted dna and separated it by molecular weight on cscl gradient in the ultracentrifuge.