BIOL207 Lecture 21: b207lect21_1.pdf
4 Dec 2014
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Experiments done on cells and structures removed intact from an organism. Example: fluorescence in situ hybridization (lecture 30: in vitro (in glass) Experiments done on individual molecules removed from an organism. Example: dna in a test tube passel. unl. edu/pages/informationmodule. php?idinformationmodule=957882007: in silico (in silicon) Example: your online genetic simulator lab: recombinant dna. A chromosome made in vivo with a new combination of alleles (lecture 4) A dna molecule made in vitro by joining pieces from two or more sources (this lecture: cloning cloning plants --> propagating seedless plants with cuttings or tissue culture (bot 382) Example: banana trees (wikipedia) cloning animals --> making an identical twin of an animal by putting one of its nuclei into an egg and growing the embryo to term (genet 302) Example: snuppy (2005) seoul national university news. nationalgeographic. com/news/2005/08/photogalleries/dogclone/ cloning genes --> making identical copies of genes (this lecture: tools needed to clone your favourite gene, overview, vector dna. Many vectors are based upon natural e. coli plasmids.
