BIOL201 Lecture Notes - Lecture 20: Spindle Checkpoint, Spindle Apparatus, Cyclin
3 Feb 2016
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Lecture 20: cell cycle checkpoints, and stem cell engineering. Provide clear summary of procedure: details about independent variable (what is the researcher manipulating to get the results), measure of dependent variable (how are they measuring the results). Instead of a paragraph: to avoid verbatim copying of materials and methods sections. Each arrow: o min, 1 min, 5 min, 10 min, 15 min. Harvest lyse cells -- (everything from then on is about measuring dependent variable) Add antibody: anti-phospho-mapk (to immunoprecipitate active mapk) (draw antibody y) (don"t say add beads etc, ect just say what you"re immunoprecipitating) True or false: growth factors regulate the growth and division of unicellular organisms (ture) Textbook never states this, microbial growth cycle (unicellular) does respond to growth factors. Growth is limited by nutrients that control the rate of growth (nutrition is a key regulatory factor) but growth factors are also involved.
