BIOCH330 Lecture Notes - Lecture 4: Heterogeneous Ribonucleoprotein Particle, Mobile Genetic Elements, Snrnp
Document Summary
Reaction can be done under native conditions. The reaction is loaded directly onto the gel so that rna complexes are not disrupted. Large smear at rst and then resolves over time. H: association os set of proteins (hnrnp: heterogeneous nuclear ribonucleoprotein protein) with the rna. When this experiment was performed the time course can be observed. Can be inferred that these complexes have something to do with splicing. A: u1 and u2 snrnp (bulging of a) C: mature catalytic spliceosome (u2, u6 and u5 since u4 has been knocked o ) U1 snrnp associates by virtue of a base pairing interaction between u1 snrna and and 5" splice site. Gu is the only universally conserves sequence at 5" splice site. Fold into a structure that autocatalysis its own excision from rna. But in addition, there are open reading frames (orf) contained within the structure. The excised intron codes for several activities and those include *dna endonuclease ,