BIOCH330 Lecture Notes - Lecture 6: Adenosine Deaminase, Adarb1, Adar
29 Apr 2019
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Not always 1:1 correspondence between genome sequence and rna. Dsrna was unwound in presence of oocyte extracts xenopus. Adenosine to inosine conversion in rna (both look very similar) Inosine looks like guanosine but lacks nh2. I is read as g by many interacting factors. Sequestering splice site in secondary structure causes editing to occur. This editing chnage occurs in over 95% of the branch sites. Adar protein: (adenosine deaminase that acts on rna) In humans there are 3 of these. Adar 1 , adar 2: in all cells. There is a regular consensus sequence cag near 3" splice site. But upstream of hat there is a caa (47 nucleotide upstream) That caa in high concentration of adar2 becomes deaminated to become cai. Since i is read as cag, we have essentially created a new 3" splice site. This is a circular rna that affects patients with hepatitis b infection. Hepatitis delta ( contains an open reading frame.
