BIOCH330 Lecture Notes - Lecture 7: Polynucleotide Phosphorylase, Polylysine, Inosine

• School: University of Alberta
• Department: Biochemistry
• Course: BIOCH330
• Professor: Andrew Macmillan

Document Summary

Look at the initial slides for lecture 6. Poly(g)->produced no protein (technically it was later discovered that poly(g) spontaneously formed tetraplexes that cannot be bound by ribosomes. Varied the concentration of c (c less) Now if you do the analysis of the polypeptide produced, you will get different proportions of different amino acids. This experiment only tells the composition of the codon but not the sequence. 3 letter codes have 64 possible combinations but only 20 amino acids. *aug: initiation codon - methionine begins polypeptides. The difference between the codons hat specify the same amino acid is usually in the 3" position. There must be more than 1 trna associated with some of the amino acids. Also some trnas can recognize multiple codons. Wobble allows some trnas to recognize more than one codon. I. e. there is not a single speci c trna for each codon. Apob gene product is an example of rna editing.