BIOCH330 Lecture Notes - Lecture 9: Puromycin, Arf1, World Federation Of Trade Unions
29 Apr 2019
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Message can be translated by multiple ribosomes on a single mrna. This is called a polysome that forms on a single mrna. Multiple peptides being formed at the same time. ** in bacteria, transcription and translation are coupled. ** protein synthesis is inhibited by antibiotics and toxins. But their dna polymerases are more similar to eukaryotes. Complexes with trna to bring them to a site of ribosome. Arf1 (release factor) = mimic of trna - allows it to sit on the speci c site at stop codon. Except, puromycin contains methyl group (ch3) instead oh oh of tyronsine. Also contains methyl instead oh oh in the adenosine base. ** amide linkage instead of ester linkage from sugar to aa. Once it reacts with the aminoacyl grip at the p site, it cannot continue in bond synthesis. Because we have amide linkage instead of ester. Amide linkage is far more resistant to attack by nucleophile than the ester linkage.
