BIOCH200 Lecture Notes - Lecture 17: Drug Design, Nucleophile, Heme
30 Oct 2016
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Biochem 200 - lecture #17 - enzymes pt. The speed of a favourable biochemical reaction is determined by the size of the. Enzymes reduce the free energy of the transition state: enzymes do not affect the free energy change ( g) of the reaction. Instead, enzymes help to achieve the transition sate. After reaction, enzyme will be released intact. How do enzymes reduce the free energy of the transition state: removing substrates from the aqueous solution (aka desolvation) So can avoid substrate interacting with h2o: proximity and orientation effects. For the reaction to proceed successfully, need to have the right conformation and orientation: taking part in the reaction mechanism, stabilizing the transition state. Active sites: does not constitute the entire enzyme, but is a subset of the enzyme. Constitutes a small portion of the protein: key amino acids are usually located in the active site. Catalysis: active sites determines affinity, specificity and rate of reaction.
