CHEM 3400 Lecture Notes - Lecture 1: Gas Chromatography, Partition Chromatography, Elution
4 Apr 2016
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Mobile phase: solvent moving coninuously through the column. Diference in mobility through staionary phase causes components to separate into bands/zones. Chromatogram is a plot of signal intensity versus ime. Solutes elute from the column based on how much are retained by the staionary phase. Adsorpion chromatography - solid staionary phase binds analyte based on polarity. Normal phase - polar solid material, non-polar mobile phase. Reverse phase - non-polar solid material, polar mobile phase (more common now) Pariion chromatography - liquid staionary phase bonded to a solid. Two factors inluence eiciency of separaion - diference in relaive retenion ime, width of peaks. Asymmetric bands can appear if the column has been overloaded (froning peak) or if there is uneven retenion from the column (tailing peak) Gas chromatography - the analyte is a gas that is transported through the column by a carrier gas. Sample is injected into a heated port where it is vaporized.
