MBB 463 Lecture Notes - Lecture 6: Exponential Growth, Shotgun Sequencing, Applied Biosystems
22 Jul 2020
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Document Summary
A knowledge of sequences could contribute much to our understanding of living matter sanger, 1980. Why sequence dna: phenotypes are determined by dna, but not all are compatible with health, knoqing th sequence/genotypes of our polymorphisms allows us to understand and predict health and disease. Applications of dna sequencing: plant & animal bio, diagnosing diseases & predicting treatment efficacy, evolutionary biology, understanding the origins of phenotypes within species. History: goal is to know order of nucleotides in a sequence @ high confidence, 3 generations since 1973. Pcr: traditional pcr, dntps used to create a copy be elongating a strand, dntp has hydroxyl group which attacks the phosphate backbone on the new dntp this allows it to extend this facilitates the addition, 3 steps. Includes: dna, primers, dntp: step 1, denaturation (dsdna denatured into ssdna, step 2, annealing (primers bind here, step 3, elongation from the primer.
