MBB 342 Lecture Notes - Lecture 2: Sanger Sequencing, Chloroform, Solvent
18 Nov 2020
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Sequencing a genomw: extract genomic dna, put it into a library, sequence it, assemble reads, annotate sequence. Too acidic or too alkaline and dna will start to degenerate this is also why carbs are removed: to remove rna: add rnase (probably after lysis, to only isolate mitochondrial dna: subcell fractionation. Lacz operon die: the cut in the dna of the bacteria is right in the lacz operon, the inserted dna must contain this operon so that the bacteria can be regularly functioning. Cloning: colony: millions of identical bacteria that came from the same progenitor, 2 types of clones, bac (bacteria artificial chromosome) Databases: 2 types, primary, raw data, given an accession number, this never changes, but the version of it is changed, cite accession and version in papers, secondary, review all this shit. Lecture 3: ngs, applications, emergency genomics, sars virus, anthrax contamination, new flu strains, honey-bee epidemic,
