MBB 231 Lecture Notes - Lecture 4: Methylene Blue
Document Summary
All microscopes require 3 main elements for the formation of an image: a source of illumination, a specimen to be examined, a system of lenses to focus the illumination on the specimen. A microscope functions in detecting, magnifying, and resolving small objects. Magnification: enlarges an image, but cannot distinguish between 2 specific points. Resolution: the minimum distance that can separate 2 points. Increasing the resolving power allows for two points that are in close distance to one another to become. There are three major factors that influence the resolving power of a microscope: the wavelength. Wavelength: the distance from one crest of one wave to the crest on the next wave. A longer wavelength can be disrupted by a large object and therefore making that object visible. Decreasing the wavelength will allow smaller objects to disrupt the wave and make the object. Angular aperture: the half angle (a) of the cone of light entering the objective lens from the.