BISC 202 Lecture Notes - Lecture 11: Wild Type, Ornithine, Arginine
4 Jan 2019
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When 2 different genes are mutated, the 2 mutant strains will produce wildtype offsprings when mated together (all offspring have a wildtype dominant allele) Complementation test: a test to see if 2 mutations affect the same gene. Mate 2 pure-breeding recessive mutant strains together, see if the offsprings are all wildtype. Yes > complemented > the 2 mutations affect 2 different genes. No > failure to complement > the mutations affect the same gene. Complementation group: a collection of strains that have non-functional mutant for the same gene. Indicates failure to complement > mutation on the same gene. + indicates complementation > mutation on different gene. Look at w1 row: w1, w2, w7 are in the same complementation group (failed to complement with w1) Already assigned w2 and w7 to group #1 > skip rows w2 and w7. Look at w3 row: w3, w4, w5, w8, w9 are in the same complementation group (failed to complement with w3)
