BISC 101 Lecture Notes - Lecture 4: Polymerase Chain Reaction, Genetically Modified Food, Sickle-Cell Disease
21 Nov 2012
School
Department
Course
Professor
Document Summary
Genes are passed on from one generation to the next. 23 sets of chromosomes = 46 (humans). Cystic fibrosis (defective transport protein) -> involved in the transmission of chlorine ion. Genetics : the study of genes in populations and generation. Identifying specific individuals based on their dna sequence. Every person has a unique dna pattern. Used to solving murder cases, crime cases, paternity cases. Steps to dna fingerprinting: extract dna, cut with restriction enzymes, dna bank established, recognizes a specific sequence and cuts the dna. Rflp = restriction fragment length polymorphism: polymerase chain reaction (pcr) To amplify dna from very small samples. Dinosaur dna: gene cloning and recombinant dna, isolate gene of interest, transfer gene to another organism. Express a unique protein in an organism (e. g. Insert and express a new (foreign) gene, Human gene -> bacteria: cloning of organisms. Sperm and egg cells): animals need to create an embryo from a somatic cell, gene therapy:
