BCH 261 Lecture Notes - Lecture 5: Deoxyribonucleoside, Deoxycytidine Monophosphate, Deoxyguanosine

• School: Ryerson University
• Department: Biochemistry
• Course: BCH 261
• Professor: Costin Antonescu

Document Summary

Ribonucleotides: (have 2 oh groups on the pentose) Deoxyribonucleotides: (has 1 oh group, one h on the pentose) Hydrolysis of polynucleotides to nucleotides is a thermodynamically favoured process. Metastability: thermodynamically favoured to break down, however do so very slowly unless catalysed. The synthesis of polynucleotides involves the energy rich ribonucleotides or deoxyribonucleoside triphosphates. The monomers used for nucleic acids are deoxyribonucleoside triphosphates which after pyrophosphate cleavage, get added as deoxyribonucleoside monophosphates. A (cid:374)u(cid:272)leotide is added to the (cid:1007)" e(cid:374)d of a(cid:374) e(cid:454)isti(cid:374)g (cid:272)hai(cid:374) Formation of a phosphodiester bond results in release of pyrophosphate (ppi) The phosphodiester bond and the polynucleotide chain are directional: e(cid:454)posed (cid:1009)" a(cid:374)d (cid:1007)" e(cid:374)ds. Al(cid:449)a(cid:455)s (cid:396)ead a(cid:374)d (cid:449)(cid:396)ite se(cid:395)ue(cid:374)(cid:272)e f(cid:396)o(cid:373) (cid:1009)" to (cid:1007)" e(cid:374)d. The discovery of dna as the biomolecule for storage and transmission of genetic information: 2 different types of bacteria: (cid:862)s(cid:373)ooth(cid:863) o(cid:396) -strain is pathogenic, rough or r-strain is non-pathogenic. Experiments that showed dna to be the genetic substance: