MICR 270 Lecture Notes - Lecture 5: Cold Chain, Toxoid, Epitope
15 Apr 2018
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Document Summary
Based on the principle of the antigen-antibody interaction. Detect substances such as peptides, proteins, antibodies, hormones etc. Measures a colored reaction produced by absorbance (using a spectrophotometric plate reader) correlating with the presence of and antibody/antigen. The primary antibodies, if present, will bind to the antigens attached to the bottom: washed again, an enzyme-coagulated secondary antibody. This secondary antibody will bind to the fc portion of the primary antibodies: washed again, substrate of the enzyme attached to the secondary antibody is added to the well. The reaction of the substrate and the enzyme produce a colored product which can be measured by absorbance. Method of detecting and quantifying different cell types in a mixed cell suspension (ex. Count # of cd4+ t-cells in cases of aids) Used to determine complete blood counts (cbcs) Narrow stream of cells in single file is passed through a laser light source.
