BIOL 102 Lecture Notes - Lecture 5: Scanning Electron Microscope, Electron Microscope, Optical Microscope
Document Summary
Biol 102- lecture 5- chapter 6: tour of the cell: It is the ratio of the size of an image produced by a microscope to its actual size. At high magnifications (>1000x) precise details are not clearly visible. It is the measure of how clear the image produced by microscope is. It is also the ability to observe two adjacent objects as distinct from one another. For example, without a telescope, one might see one blurry star in the sky but when a telescope is found, one gets to know that it was actually a cluster of 3 stars. Difference of brightness between dark and light areas of an image. Ability to distinguish different structures in differently lighted areas of an image. It can be enhanced using dyes or by labelling parts of the cell. It uses light for illumination: visible light is passed through specimen and then via glass lenses. Image is magnified due to refraction of lenses.