BIOL 265 Lecture Notes - Lecture 24: Transfer Rna, Ribosome, Peptidyl Transferase
15 Apr 2019
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Recognizes specific trna (nucleotide sequences) and specific amino acid (size, charge, r group) Many have proofreading ability: discriminate between different amino acids and ensure correct amino acid used to charge trna. Lecture 24: initiation of translation (bacteria), components necessary for protein synthesis. 16s rrna of small ribosomal subunit binds shine dalgarno sequence in. Initiator trna with n-formyl methionine (fmet trnafmet) mrna positions aug codon to accept initiator trna (fmet trnafmet) Lecture 24: elongation in bacteria, ribosome has 3 sites for trna binding: Aminoacyl (a site): all other trnas enter ribosome via a site. Peptide bond formation occurs in peptidyl transferase centre in large subunit of ribosome (a rrna catalyzes this reaction) Translocation occurs in the 5" 3" direction. Lecture 24: cleavage of polypeptide from trna in p site, hydrolysis of gtp of rf3 causes dissociation of termination complex. Initiation complex made up of small subunit of ribosome, initiation factors, initiator trna charged with methionine (mt-trnai.
