BIOLOGY 2B03 Lecture Notes - Lecture 4: Chloroplast, Transfer Rna, Lysosome
11 Dec 2015
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Document Summary
Bound by a single membrane continuous with the outer membrane of the nucleus. First destination for secreted proteins: free ribosomes are in the cytosol, both types are translationally active and come from the same common pool, simply cycling on and off the er membrane. Are proteins transported to the lumen of the er, immediately upon translation so that they are not exposed to the cytosol: microsomes are used to mimic er membrane. Created by disrupting the er membranes of the cell homogenization. Mimic normal er behaviour contain all of the same membrane-associated proteins: microsome membranes treated with detergent releases proteins inside microsomes. Added protease digests the proteins: right: membrane left intact and protease is added, newly synthesized proteins are protected from proteases within, newly synthesized proteins move directly into microsomes. Hypothesis: if translation is completed, transport cannot. Control: proteins synthesized in vitro in the presence of occur microsomes: mature proteins found inside microsomes.
