BIOLOGY 1A03 Lecture Notes - Lecture 12: Rna Interference, Ubiquitin, Small Interfering Rna

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Rosada Silva

Document Summary

In situ studies: identifying which embryonic cells/tissues express a gene: analysis: where corresponding mrna can be found during development, mrna can be detected with a complimentary probe that has a fluorescent tag. In situ hybridization: complimentary probe to mrna binds in a complimentary manner to the target mrna molecule. Dna microarray analysis: in vitro; possible to examine the expression of thousands of genes at once: glass slides set up with spots containing known dna sequence, dna molecules act as probes to detect gene expression. Preparing cdna: isolated mrna serves as template for complimentary cdna facilitated by reverse transcriptase enzyme, fluorescent nucleotides are utilized and become part of the new cdna molecule, fluorescence analysis of gene activity: To stop gene expression; mrna should be degraded. Mrna stability can be regulated by: mirna: form hairpin loops that are processes into smaller single stranded mrna fragments activates rna interference machinery processes mirna is incorporated into rna induced silencing complex (risc)