BIOLOGY 1A03 Lecture 17: Lecture 17

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Rosa Da Silva

Document Summary

Thursday, march 12, 2015: we can then run it through a gel and by length can determine the template strand. Phase 2: assembly: assembly of overlapping dna sequences, contig: consensus sequence (sections of sequence overlap) Phase 3: annotation: six reading frames for any double-stranded dna sequence, important because it allows you to gure out which is the best possible components for looking at eh sequences, only one reading frame codes for proteins ex. Dna repair: occasionally there are some problems, detected during checkpoints. Mismatch repair: identifying a mistake, a repair enzyme rst breaks the backbone some distance away from mistake, then another enzyme come along and removes successive nucleotides, including the one with mismatched base. Thursday, march 12, 2015: other enzymes close the gap by new dna synthesis, using the intact dna strand as a template. Mutations in the nucleotide sequence: a point mutation: speci c nucleotide non mutant, synonymous, non-synonymous.