BIOLOGY 1A03 Lecture Notes - Lecture 14: Dna Replication, Primase, Chromosome

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BIOLOGY 1A03 Full Course Notes
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BIOLOGY 1A03 Full Course Notes
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Watson and crick followed up on their paper about how dna has a double-helix structure: Dna consists of a pair of complementary template chains with h bonds that are broken before replication to allow for unwinding and separation. When dna is copied, each strand serves as a template for a new strand to form two new helices with one strand being old and one being new. (semiconservative model) Dispersive theory: the two parental strands would somehow get back together after replicating, all four strands combine into a mixture of old and new strands. Meselson and stahl proved that replication is semiconservative: They cultured e. coli bacterial cells in a medium with nucleotide precursors with the radioactively labeled heavy isotope of nitrogen (15n) They transferred the bacteria into a medium with 14n. Every new strand of replicated dna would be built containing 14n rather than 15n isotopes.

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