BIOLOGY 1A03 Lecture 9: Theme 3- module 4.docx

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Rosada Silva

Document Summary

Theme 3: responding to the environment module 4: turning off the signal. Learning objectives: examine how in situ analysis can reveal spatial and temporal changes in the gene expression of an organism. In situ studies of gene expression: ex. Suspect that a specific gene plays an important role in the embryonic development. It is possible to identify which embryonic cells or tissues express the gene by undertaking an analysis as to where the corresponding mrna that is transcribed from that gene can be found during development in the intact organism. The mrna can be detected with a complementary probe that has a. In situ hybridization fluorescent tag attached to it. Dna microarrays examine the expressions of thousands of genes at once: based on the base-pair interactions and binding of complementary strands of nucleic acids, glass slides that have tiny spots containing a known dna sequence or gene.