BIOLOGY 1A03 Lecture Notes - Lecture 4: Hiv, Glycosyltransferase, Blood Vessel

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crimsonbat46

3 Sep 2018

School

McMaster University

Department

Biology

Course

BIOLOGY 1A03

Professor

Alastair Tracey Rosa Da Silva

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BIOLOGY 1A03 Full Course Notes

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While every human cell in our body carries the same genome across 23 chromosome pairs, there are a great deal of variations that can be found in our genome. Evolution has largely led to alternate alleles or variations of specific genes. While there are 2 alleles of a gene per person, there can be many alleles or different haplotypes n a population. This has led to genetic variation across individuals & populations. But while small variations in dna sequence can lead to genetic variation it is the expression of those genes w in the genome that produces cell type specific proteomes that are important for vital cellular processes. W in multicellular organisms, the cellular proteomes are driven by different transcriptional programs that are able to direct different ells to engage in specific functions in association w other cells or even w in tissues.

Related Questions

Question 1

1 pts

Cystic fibrosis is caused by nonsense and missense mutations in the CFTR gene, which encodes for a chloride channel. You are studying cystic fibrosis patients to determine what mutation they possess in the CFTR gene. The difference between the mutant and wild type CFTR genes can be uncovered by examining the CFTR:

	DNA
	mRNA
	protein
	tRNA

Question 2

1 pts

You decide to identify the CFTR mutation by analyzing the genomic DNA of your patients compared to healthy individuals. You specifically are looking to see whether a specific 3' gene truncation has occurred in the patients. You will determine this using hybridization techniques with samples from healthy and CF patients. Which of the following will allow you to accomplish this?

	Using an RNA probe complementary to the region not removed by the truncation.
	Using an RNA probe complementary to the region removed by the truncation.
	Using an DNA probe complementary to the region not removed by the truncation.
	Using an DNA probe complementary to the region removed by the truncation.

Question 3

1 pts

You would like to ensure that this experiment (to determine whether patients have a specific CFTR gene truncation using hybridization) is properly controlled. Which of the following samples must you test?

	The genomic DNA of a healthy individual who does not have cystic fibrosis.
	The genome of a CFTR patient known to have the specific truncation you are trying to identify.
	The genome of a CFTR patient with a missense mutation but full length gene.
	The genome of a healthy individual married to a CFTR patient with the specific truncation you are trying to identify.
	The genome of a patient with muscular dystrophy, which can be due to a trucation in the dystrophin gene.

Question 4

1 pts

To conduct the hybridization experiment, you are trying to decide between using a DNA or RNA probe. Which would be ideal to use and why?

	As both are composed of nucleic acids, using either would result in identical results.
	An RNA probe because RNA has uracil bases.
	An RNA probe because it could also be used in a translation experiment.
	A DNA probe because it is more stable than RNA.
	A DNA probe because RNA cannot bind to DNA.

Question 5

1 pts

Which of the following will lower the Tm of a given DNA strand?

	Increasing the percentage of GC base pairs.
	Raising the pH of the solution from neutral to basic.
	Decreasing the buffer concentration from 50mM NaCl to 5mM NaCl.
	None of the above.

Question 6

1 pts

One step of the Hershey/Chase experiment involved blending the virus/cell mixture before centrifugation and probing the pellet for radioactivity. Why was the blending step necessary?

	To collect the bacteria at the bottom of the tube.
	To break open the bacteria to release the genome.
	To separate the bacteria from the bacteriophages.
	To be able to detect the radioactivity.

Question 7

1 pts

Imagine Hershey/Chase had used an RNA virus (genome composed of RNA) instead of a DNA virus in their experiment. Would radioactivity still have been found in the pellet?

	No, because only DNA can be labeled with radioactivity.
	No, because the RNA genome would not enter the bacteria upon infection.
	No, because while DNA and RNA nucleotides are similar, they are not identical.
	Yes, because DNA and RNA nucleotides are similar.
	Yes, because genome in any form (DNA, RNA, protein) would be labeled similarly.

Question 8

1 pts

Griffith and Avery's transformation experiments allowed us to identify that DNA is our genetic information. Which of the following scenarios would result in bacterial cells that are capable of killing mice upon injection?

	Heat killed non-virulent bacteria is added to a live virulent bacteria strain.
	Heat killed virulent bacteria is added to a heat killed non-virulent bacteria strain.
	A heat killed virulent bacteria that is treated with a nuclease, is then added to a non-virulent bacteria strain.
	Heat killed mouse cells are added to a non-virulent bacteria.

Question 9

1 pts

The human genome consists mostly of non-coding DNA. Which of the following are benefits of this?

	Random DNA mutations generally won't affect RNA and protein function.
	It is faster to duplicate the genome when these are present.
	The existence of introns can lead to multiple variations of proteins encoded by a single gene.
	It is unlikely transposons would exist in the genome if there was too much protein coding DNA.

Question 10

1 pts

Andrew Murray's sister, Andrea, is adding to her brother's work on chromosomes. She is using cells that are unable to synthesize adenine (âade) and histidine (âhis). The plasmid she is currently working with consists of an origin of replication and the Ade gene.

Following her transformation of the plasmid into her yeast, what media will the cells be plated on to select for cells that have picked up the plasmid?

	Media containing histidine
	Media containing adenine
	Media lacking adenine
	Media lacking histidine

tealcockroach755

1. Humans have the scientific name Homo sapiens. We are members of the family Pongidae, in the order Primates, in the class Mammalia, in the phylum Chordata. Based only on this information, which of the following is not a correct statement?

a. The speciation event (lineage-splitting) that generated the genus Homo occurred earlier than the speciation event that generated the species H. sapiens.

b. The most inclusive of these taxonomic groups is Chordata.

c. The genus Homo might have as many species as are in the entire family Pongidae.

d. There can be no other species in the genus Homo.

e. All mammals must be Chordates.

2. Endotherms differ from ectotherms in that:

a. Environmental temperature has little effect on the energy budget of endotherms but a strong effect on the energy budget of ectotherms.

b. An endotherm's metabolic rate is not affected by temperature whereas the metabolism of an ectotherm has a Q10 of 2-3.

c. Endotherms are vertebrates and ectotherms are invertebrates.

d. On average, endotherms are much better sprinters (i.e., can run faster for short distances) than ectotherms.

e. Endotherms are much better able to support endurance exercise than ectotherms.

3. You measure oxygen consumption of a lizard at three temperatures, as follows: 18 Â°C: 0.090 ml O2/min 23 Â°C: 0.135 ml O2/min 28 Â°C: 0.200 ml O2/min From these data you calculate the Q10 to be:

a. 1.8

b. 2.0

c. 2.2

d. 2.5

e. none of these are correct.

4. What is the best description of how evolution by selection (natural or artificial) works?

a. mutation generates variation randomly, sex makes new combinations of genes randomly, and selection picks the best ones for reproduction.

b. selection leads to non-random mutation to make useful new alleles, which are then combined by sex.

c. exposure to environmental factors leads to non-random mutations that improve fitness.

d. through selection, mutations occur in those genes that will improve fitness.

e. mutation generates random new alleles, and sex combines them in ways that select the best for future generations.

5. Which of the following is a correct match between a major lineage and its characteristics?

a. Archaea: extremophiles, ribosomes, RNA as genetic material, no nuclear membrane

b. Eukarya: mitochondria, DNA as genetic material, photosynthetic or chemohetrotrophic

c. Bacteria: mitochondria, DNA as genetic material, no nuclear membrane

d. Eumetazoa: extremophiles, ribosomes, DNA as genetic material, nuclear membrane, chemoheterotrophic

e. Protostomes: triploblastic, eucoelomate, blastopore becomes anus, indeterminate cleavage

6. Which of the following statements about animal life history events is correct?

a. Being multicellular requires a larval stage in the life cycle.

b. Mutation is sufficient to produce considerable genetic variation between different tissues in an individual.

c. Senescence is defined by slow, constant decline in survival as individuals get older.

d. Different cell types are formed when different but specific portions of the zygoteâs DNA are lost from the different germ layers during development.

e. In an individual, the only cells that differ genetically from other cells are gametes.

7. Which of the following statements about terrestrial vertebrates is correct?

a. Jaws evolved from paired appendages, which in turn evolved because they improved swimming ability.

b. Legs evolved from fins after early fishlike vertebrates invaded the land.

c. Jaws and inner ear bones evolved from gill arches, and leglike appendages evolved under selection for aquatic locomotion.

d. Viviparity (giving birth to well-developed young instead of laying eggs) was vital to the ability to reproduce on land without returning to the water.

e. Lungs for breathing air evolved after early fishlike vertebrates invaded the land (the first land vertebrates had to depend on gills for breathing air)

8. The essential features of a hydrostatic skeleton include:

a. antagonistic muscles.

b. a non-deformable fluid-filled space

c. flexible joints.

d. compressible material capable of changing volume

e. all of the above (a) â (d) are correct.

9. You are a Peace Corps volunteer physician in a developing country. You notice that some of the villages you visit have a high incidence of a parasitic disease, but other nearby villages are unaffected. Investigating you find the villages with disease are in wet areas with lots of snails and frogs, while the disease-free villages are in drier places but have lots of insects and mice. From this information, what might you reasonably conclude about the type of parasite responsible for the disease?

a. Itâs a trematode (these need mollusks as intermediate hosts).

b. Itâs a nematode (these are vectored mainly by frogs).

c. Itâs an apicomplexan parasite (these require wet areas and enter the skin through standing water).

d. Itâs a trypanosome (these are transmitted by mammal bites).

e. The information doesnât provide useful clues about the type of parasite.

10. Which of the following pairs of primary diagnostic characters is correctly matched with taxonomic groups?

a. circulatory system vs. water vascular system: Nematoda vs. Echinodermata

b. schizocoely vs. enterocoely: Radiata vs. Bilateria

c. pharyngeal slits vs. polyp and medusa: Chordata vs. Cnidaria

d. gastrovascular cavity vs. complete gut: Cnidaria vs. Cestoda

e. hemocoel vs. contractile vacuole: apicomplexans vs. rhizopoda

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BIOLOGY 1A03 Lecture Notes - Lecture 4: Hiv, Glycosyltransferase, Blood Vessel

BIOLOGY 1A03 Full Course Notes

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