BIOCHEM 3H03 Lecture Notes - Lecture 13: Chronotype, Membrane Transport Protein, Cortisol
19 Apr 2019
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Document Summary
Levels outside the normal ref range are associated with pathology. An elevated cortisol level can be the result of a host of underlying biochemical disturbances. Physiological factors to causing outside of normal range. Biological variation: ubiquitous throughout all of the topics, age, gender, ethnicity, diurnal variation, stress, different in the morning than at night, depends on the time of day. Pre-analytical and analytical factors (artifacts) how is the sample collected/treated (ex. Measuring cortisol but the patient is very anxious as blood is being drawn) Assay interferences: might be certain drugs on board that will change cortisol levels physiologically or interfere with the assay chemically. Collection condition: standing vs sitting when sample taken, anxiety level when sample was taken. Problems with quality control (qc: instrument not performing correctly. Selection: any method that allows to pull-out your molecule of interest. If turnaround time is too long, test is useless: the accuracy and precision required, e. g.
