PHGY 550 Lecture Notes - Lecture 3: Bone Resorption, Wnt Signaling Pathway, Fibroblast Growth Factor 23
31 Mar 2018
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Document Summary
Unloaded leads to bone resorption and bone loss; however can be reversed; i. e. astronaut loses up to 20% of bone mass in pelvis and legs after 3/4 months; reverses within 30 60 days after landing i. e. paraplegic patients lose 25% of bone mass within first month months; reverses within 30 60 days after landing i. e. paraplegic patients lose 25% of bone mass within first month i. e. bedrest patient; looking for ways to prevent loss of bone in these patients. Mechano sensing via fluid flow shear stress perturbs: tethering elements between canalicular wall and cell membrane, cell body, primary cilia d. Conventional histology and immunochemistry (ihc: ihc used to visualize the factors that are expressed; i. e trap staining in osteocytes, staining used to see bone formation over time; i. e fluorochrome labelling for, staining used to see bone formation over time; i. e fluorochrome labelling for mineral apposition rates (mars)
