PHAR 505 Lecture Notes - Lecture 13: Mother Liquor, Ammonium Sulfate, Supersaturation
13 Nov 2017
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Document Summary
It is by far the most widespread of the structural biology methods (for structure determination) Remember that nmr is used not only for structure determination but for ligand:receptor interactions and characterisation of small molecules. This method gives us direct insight into the structure. We get electron density maps that tell us exactly where the atoms are. Electron microscopy is the same resolution is very good (and getting better over time) Prof wants to give us a taste of what xrc entails: what to do to get a protein structure. Compared to other methods, it is a lot more solid: gives pretty strong evidence. Step 1 crystallisation: crystals of macromolecules with or without ligands are grown, approx. Step 2 single crystals are mounted on xr beam to collect diffraction data: we end up seeing spots with different intensities. Step 3 the atomic structure is determined from the xr diffraction data: contour maps can help us to build models.
