MIMM 211 Lecture Notes - Lecture 10: Petri Dish, Serial Dilution, Methylene Blue
18 Oct 2016
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Lec 10: some techniques, bacterial genome replication + gene expression. Gas generator envelope: add water to generate h2 and co2. Catalyst chamber with palladium pellets: 2h2 + o2 2h2o, neutralize o2. Anaerobic indicator strip: methylene blue becomes colourless in anaerobic conditions: centre: stationary phase or death, because nutrients can only diffuse so far in, surface/edge: actively growing (exp phase). Aerobic conditions if petri dish is in o2. Liquid: all cells are in same phase of growth. 9ml pure medium again, over and over again to achieve enough dilution: plate the last diluted sample, count the bacteria under microscope, con: takes a long time, can only get results the day after. If you solve for n (amount of doublings), log both sides: n = 3. 3(log nt log no) Griffiths transformation exp: s = smooth strain, pathogenic to mice, r = rough strain, harmless and nonvirulent.
