LSCI 211 Lecture Notes - Lecture 12: Dihydrolipoyl Transacetylase, Pyruvate Dehydrogenase, Thiamine Pyrophosphate
28 Aug 2016
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Document Summary
Very permeable outer membrane, impermeable inner membrane with transport proteins. Pyruvate is transported from the cytosol into the matrix and transformed to acetyl co-a. Atp is produced and transported to the cytosol. Oxidative decarboxylation: pyruvate + nad+ + coash acetyl-coa + co2 + nadh + h+ Pyruvate dehydrogenase has 3 parts with 3 functions. Pyruvate dehydrogenase (e1)- pyruvate decarboxylation with thiamine pyrophosphate. Dihydrolipoyl transacetylase (e2)- acetyl coash transfer with lipoic acid & coash. Dihydrolipoyl dehydrogenase (e3)- re-oxidize dihydrolipoamide with nad+, fad. Outdoor camping is kinda sorta super fun man! Net reaction: acetyl-coa + 3nad+ + fad + gdp + pi + 2h2o. Potential to generate 36-38 atp from one glucose molecule. In reality, closer to 30 atp are generated due to cell inefficiencies. Citrate synthase inhibited by succinyl co-a, citrate, atp and nadh. Isocitrate dehydrogenase inhibited by atp and nadh, activated by adp and nad+ Ketogluterate dehydrogenase inhibited by nadh and succinyl co-a, activated by amp.
