FDSC 305 Lecture Notes - Lecture 5: Ninhydrin, Polyphenol Oxidase, Stereocenter
29 May 2017
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Document Summary
Eg, cyclodextrin, polyglutamates, derivatized proteins: for molecules to behave as enzymes, they must have a binding site and a transformation site. The binding site contributes groups for the binding of the substrate molecules. After substrates are bond existing bonds may break or new bonds formed to form products: the overall enzyme molecule is very large, relatively. The region where binding and transformation take place is very small and is known as the active site: enzyme active site. Substrates bind to active site and may be transformed into products. But there can be no transformation without binding: holoenzyme. Holoenzyme: protein part apoenzyme (eg, enzymes without cofactors: trypsin, pepsin, chymotrypsin) + essential non-protein part prosthetic. Examples of enzymes requiring prothetic group for activity: polyphenol oxidase, xanthine oxidase: enzyme specificity: Epimerases: enzyme specificity - isomerases: racemase and epimerase. They are all isomerase that catalyze the inversion of stereochemistry in biological molecules.
