ENVB 437 Lecture Notes - Lecture 3: Bioinformatics, Respiratory Tract, Acute Respiratory Distress Syndrome
4 Jun 2018
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Eukaryotic cells and viruses – lecture 03 (January 15th 2018)
Slide 57
- They are frertilized
- The virus is injected in the chorioallantoic membrane
- The allantoic cavity is the site of replication and
- After some time the virus is collected
- Keep the surface spikes of the more virulent virus and the rest will be made of surface proteins etc.
- This will reassert and there are 256 combinations (4!)
o You select the HA and the NA of the more virulent strains
- You have to have 3 different egg preparations (or 4 for the quadrivalent)
- Millions of eggs are required for this
o 1 egg is around 1 dose
o the egg is sterile so you work with a sterile environment for the most part
find more resources at oneclass.com
find more resources at oneclass.com
Slide 58
- You take the attenuated donor strain, reassort with the more sirulent HA and NA and have everything from the
attenuated virus
- So you have everything from the attenuated with the exception of HA and NA
x
Slide 59
Slide 60
Slide 61
- Everyone 6 months of age and older should get a flu vaccine as soon as the 2011-2012 vaccines are available
- Vaccination of high risk persons is especially important to decrease their risj of severe flu illness
- People at high risk of serious flu complications include young children, pregnant women, people with chronic
health conditions like asthma, diabetes or heart and lung disease and people 65 years and older
- Vaccination
find more resources at oneclass.com
find more resources at oneclass.com
Slide 62
- Currently manufacturers projects 170-180 million doses of flu vaccine to be produced for 2015-16 season
(US)
- Cost per vaccination???
Slide 63, 64
- Take home message is that efficacy of the vaccine is variable year-to-year
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Eukaryotic cells and viruses lecture 03 (january 15th 2018) The virus is injected in the chorioallantoic membrane. The allantoic cavity is the site of replication and. After some time the virus is collected. Keep the surface spikes of the more virulent virus and the rest will be made of surface proteins etc. This will reassert and there are 256 combinations (4!: you select the ha and the na of the more virulent strains. You have to have 3 different egg preparations (or 4 for the quadrivalent) Millions of eggs are required for this: 1 egg is around 1 dose the egg is sterile so you work with a sterile environment for the most part. You take the attenuated donor strain, reassort with the more sirulent ha and na and have everything from the attenuated virus. So you have everything from the attenuated with the exception of ha and na x.
