BIOC 311 Lecture 5: Lecture_5

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Pentose phosphate pathway is a pathway branching off glycolysis. Metabolic uses: (1) produce nadph (2) produce pentose sugars (3) utilize pentose sugars. Can be viewed in two sections: (1) oxidative steps. Stage 1: oxidative reactions (steps 1-3) (2) non-oxidative steps. Stage 2: isomerization and epimerization reactions (steps 4 and 5) Stage 3: c-c bond cleavage and formation reactions (steps 6-8) Reactions of stages 2 and 3 are reversible such that the products of the pathway vary with the physiological needs of the cell. All steps from stage 1 are irreversible, conferring directionality. Difference between nadph and nadh: both are present in cells, but nadh and napdh are not interchangeable. Metabolic enzymes that use nadh or napdh have high specificity for their coenzyme , but not the other even if both are really similar in structures. Nadh: use for catabolism => used to make atp. Napdh: use for anabolism => used for biosynthesis of fatty acids, cholesterol (lipids)

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