BIOC 311 Lecture Notes - Lecture 13: Nadh Dehydrogenase, Succinic Acid, Rotenone

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26 Mar 2020

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Complex i: feeds nadh to coenzyme q. Complex ii: feeds succinate to coenzyme q. Complex iii: processes the contents of coenzyme q into cytochrome c. Redox reactions: do not happen freely in solution (occur in proteins), they are there to protect and stabilize electron carriers and pump h+ across the membrane into ims. Etc components: arranged to increase srp in a sequential manner. We use these in order to track the order of the complexes. First, we isolate the mitochondria with an inhibitor in order to figure out what is bound to (e. g. what kind of protein) If you put a red light on sherbrooke, there will be a build up of traffic coming from. Then we test o2 consumption and atp production. When we add cyanide (cn-), no atp gets produced, so it must be last. If cn- was added first, nothing happens, no reaction no etc. P/o ratio: the amount of atp phosphorylated per o2, nadh, fadh2 (2e-)

Related Questions

19. Which of the following is a component of Succinate dehydrogenase in Electron transport chain?

A. Niacin

B. FMN

C. FAD

D. Coenzyme Q

E. Lipoic acid

20. In electron transport the flow of electrons:

A. is bidirectional

B. goes from a high energy state to a low energy state

C. takes place in the outer mitochondrial membrane

D. are used to pump H+ into the mitochondrial matrix

21. Which of the following statements about the electron transport chain is NOT correct

A. It is located in the inner mitochondrion membrane

B. Cytochrome c accepts electrons from complex II

C. Cytochrome c oxidase (complex IV) accepts electrons from Cytochrome c

D. Complex I is called NADH dehydrogenase

E. Coenzyme Q accepts electrons from complex I and complex II

22. In the electron transport chain, the enzyme cytochrome C oxidase:

A. Uses H2O as a substrate

B. Produces HOOH as a product

C. Cannot function if oxygen is absent

D. Accepts electrons from hydrogen ions

E. Uses ADP and Pi as substrates

Can you answer the following questions:

1. Carbon monoxide is considered toxic because it acts on Complex IV. How would the addition of carbon monoxide to actively respiring mitochondria affect the relative oxidation-reduction states of all components of the electron-transport chain?

A) All four complexes would remain oxidized because they function as a multisystem complex.

B) Complexes I and III would be reduced, but complexes II and IV would be oxidized because the

electrons come from FADH2 oxidation, not NADH.

C) Complexes I, II, and III would be oxidized but Complex IV would remain reduced.

D) Complexes I, II, and III would be reduced and Complex IV would be oxidized.

E) All four complexes would remain reduced because they function as a multisystem complex.

2. Electrons are not very soluble in hydrophobic environments such as a bilipid membrane. What evidence is there that electrons move from complex to complex through the lipid membrane?

A) Cytochrome C is the electron carrier in the membrane and undergoes a head-over-heel flip to set up the proton gradient.

B) Cytochrome C is the electron carrier in the membrane and contains a cydrophoric porphyrin center.

C) CoQ is the electron carrier within the membrane and seems to be confined to the respirasome.

D) CoQ is the electron carrier within the membrane and it contains a hydrophobic porphyrin center.

E) FeS clusters are the carriers within the membrane and the electrons move from one cluster to the

next in a chain-like fashion.

3. What is the rationale for saying that âelectrons flow down the electron-transport chainâ?

A) Electrons flow from oxidized carriers to reduced carriers in discrete steps like a staircase.

B) Electrons flow from half-reactions with more positive redox potentials to more negative.

C) Electrons flow from reactions that continuously generate negative free energy values.

D) Electrons flow from the outer surface to the inner surface, a top down perception.

E) Because we think of electrons flowing like a liquid, the only direction electrons can flow is down.

BIOC 311 Lecture Notes - Lecture 13: Nadh Dehydrogenase, Succinic Acid, Rotenone

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