BIOC 311 Lecture Notes - Lecture 9: Dihydrolipoamide Dehydrogenase, Pyruvate Dehydrogenase, Acetyl-Coa

• School: McGill University
• Department: Biochemistry
• Course: BIOC 311
• Professor: Maxime Denis

Document Summary

Pyruvate dehydrogenase complex (pdc): regulates the trafficking (flow) of pyruvate from the cytosol into the mitochondria. Pdc, cac, aerobic metabolism = in mitochondria: pdc converts pyruvate into acetyl-coa once the pyruvate is imported into the mitochondria, it is not a transport, acetyl-coa + oxaloacetate come together in cac. There are kinases and phosphatases that regulate this: phosphorylation inhibition, desphorylation activation, like glycogen synthase. Total reaction = pyruvate + coa + nad+ acetyl-coa + co2 + nadh. Tpp, lipoic acid, coa, fad, nad: they are all involved in the transfer of electrons in order to convert coa acetyl- Works in a cycle: 5 reactions, 5 coenzymes, 3 enzymes (e1, e2, e3): The most important part of the entire pyruvate decarboxylation reaction is the generation of acetyl-coa from pyruvate, whilst reducing nad+ to nadh where it is used in the etc, pumping ions across the membrane to make atp.