BIOC 300D2 Lecture Notes - Lecture 3: Monoamine Oxidase A, Sucrose, Biogenic Substance

• School: McGill University
• Department: Biochemistry
• Course: BIOC 300D2
• Professor: Jose G.Teodoro

Document Summary

Lecture 3: fractionation of subcellular components and the use of marker enzymes. Fractionation process will depend on the type of population you start with. Heterogeneous population (rat liver: 30 different cell types from which you need to extract organelles) vs. homogenous population (purified cell culture) Technology develops techniques that isolate one cell at a time such that you can fractionate tissues and look at cellular level (genomics, transcriptomics). E. g. each cancer cells will have different genotype since dna mutagenic rate is high. Other isolation techniques besides centrifugation: magnetic beads: based on antibody recognizing markers at surface of cell. For instance, you could have an antibody against receptor protein which will have metal ion on it. When fraction mixed with antibody, specific population will be recognized by this antibody and a magnet will bind the cells that bound the metal antibody.