ANAT 262 Lecture Notes - Lecture 2: Nuclear Localization Sequence, Lamin B Receptor, Nummelan Palloseura
30 Aug 2016
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Document Summary
Signal recognition particles (important to secrete proteins) assembled in nucleoli. Nucleoli not only make small & large ribosomal subunits, also assemble signal recognition particles. Know hutchinson"s gilford, not expected to know all names of diseases. Know lamin a mutations can affect different tissues. Npc transport is gated, moves material in/out of nucleus, both directions. 3 major nups families: fxf repeats: repeats of 3 aa residues (phenylalanine, any aa, phenylalanine) (phenylalanine are hydrophobic) e. g. include nup358, 214, 62, 153 important for protein trafficking across npc. #s = molecular mass of pore (in thousands: glfg repeats (glycine, leucine, phenylalanine, glycine) e. g. nap 98 rna export, no repeats anchor npc to membrane. Trafficking across npc occurs at central gated channels (black hole) Diffusion & active transport, not everything goes through npc. Filaments stick to central gated channel (not empty), repeats penetrating into central gated channel. Middle of npc: several nups that build substructures (modules)
