ANAT 262 Lecture Notes - Lecture 8: Golga2, Mannose, Cisterna

• School: McGill University
• Department: Anatomy & Cell Biology
• Course: ANAT 262
• Professor: Ursula Stochaj

Document Summary

Motor proteins on microtubules selective for direction of transport. Small gtpases use gtp to be active and on membrane; hydrolyze to gdp inactive and in cytoplasm. Sec12 gef on donor membrane recruits sar1 sar1"s hydrophobic tail inserts into membrane (gtp-bound) Sar1 recruits sec 23/24 heterodimer (recognizes transmembrane cargo/cargo receptor for lumenal) & sec 13/31 tetramer (curves membrane, buds) Sec 23/24 hydrolyze sar1 gtp to gdp structures not stable on membrane uncoat. Vamp2 involved in synaptic vesicles on vesicle membrane. Vsvg protein mutated to be temperature sensitive, can create temperature block (15 degrees) to freeze proteins, releasing temperature block resumes movement. Cis faces er, trans faces lysosomes, pm, secretory vesicles. Cis golgi network & trans golgi network responsible for sorting. Endo h resistancy test: determine which stage of pathway. If sensitive to endo h enzyme not yet completed. If resistant to endo h reaching complex oligosaccharide. Mannoses sensitive to endo h, modification of oligosaccharides blocks endo h from reaching mannose.