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ANAT 262 Lecture : 2.16 notes (1).doc

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plumcod665
21 Aug 2012
School
McGill University
Department
Anatomy & Cell Biology
Course
ANAT 262
Professor
John Presley

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Document Summary

Golgi apparatus: items enter from er on the cis-golgi, next is the medial golgi and then trans-golgi (also called tgn = trans golgi network) Increases in acidity towards the trans golgi (trans = 6. 5 ph) Various golgi enzymes are localized to different compartments of the golgi. Cisternae are detaching and peeling off the tgn. Stacks consist of layered cisternae (like stack of pancakes) Golgi enzymes (most notably those that modify n-linked oligosaccharides) are local- ized sharply to particular regions of the golgi. Stacks are arranged in long ribbons with disorganized areas between the stacks. Functions of the golgi: sequential modifications of n-linked oligosaccharides. Glucose is trimmed off in er, and sugars are added in the golgi (ends with negative charge), different enzymes must work in sequence in the golgi. Functions occur at different places in the er: o-linked glycosylation and proteoglycan glycosylation.

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Related Questions

1. How do viral proteins get localized to the nucleus?

2. How do viral proteins get localized to the plasma membrane?

3. How does a nascent protein get targeted to the ER?

4. What amino acid residue on a target protein is attached to the sugar?

5.Where in the cell are N-linked oligosaccharides FIRST added to a glycoprotein?

6.Where in the cell does the initial sugar get processed into its mature form?

7. That is, which exact compartment(s) of the secretory pathway is mannose removed? Galactose added? Sialic acid added? How does the position of glycosylation (N-linked vs internal) affect which chaperone is used to properly fold proteins in the ER?

8. What compartments comprise the secretory pathway in mammalian cells?

9.How do proteins get transported from the ER to the Golgi?

10.How do the transport vesicles return from the Golgi to the ER?

11.What happens to their components when they get there? How does poliovirus inhibit the secretory pathway?

SHORT answers please

33 Different glycoproteins can have a diverse array of oligosaccharides. Which of the statements below for making glycoproteins on plasma membrane is true?

(a) Extensive modification of oligosaccharides occurs in the extracellular space.

(b) Initial oligosaccharide chains are made by addition of one sugar at a time onto the protein in the ER lumen.

(c) Each layer of Golgi membrane structures contains identical modification enzymes for modifying sugar chain on proteins.

(d) ER lipids can be modified by sugar chain, which can be used for sugar chain modification of the proteins.

Regarding Golgi specialized functions:

a) Discuss the Golgi’s roll in ‘trafficking” cell components (include discussing of the ‘two faces’)

b)What role does dolichol phosphate play in glycosylation?

c)Does glycosylation occur before or after a protein is folded?