ANAT 261 Lecture Notes - Lecture 99: Paraffin Wax, Propylene Glycol, Osmium Tetroxide

• School: McGill University
• Department: Anatomy & Cell Biology
• Course: ANAT 261
• Professor: Craig A.Mandato

Document Summary

Longitudinal section increase the amount of ethanol gradually. You remove alcohol to allow infiltration with paraffin remove the wax by using xylene remove xylene by use a rotary micrometer to cut the specimen. Chemical fixation by using formalin stop the biochemical reactions. Dehydration by ethanol to prevent shrinkage end up with 100% ethanol and no formalin & water. Clearing with xylene (or paraffin solvent) wax by adding xylene gradually. Infiltration 50% xylene/50%paraffin; tissue infused with paraffin wax to basically remove cassette containing specimen from tissue processor. Put wax+tissue in cold water=solidification (embedding 100% paraffin). Preparation for staining rehydration we want water through descending alcohol (reduce gradually the amount of alcohol). **instead of formalin (in chemical fixation), use freezing. ****instead of formalin, use glutaraldehyde & osmium tetroxide. ***(for embeding) -for dehydration, use ethanol -for clearing, use propylene glycol -for filtration, use propylene glycol/plastic. *instead of h&e, use lead citrate &/or histochemical reaction.