ANAT 261 Lecture Notes - Lecture 4: Stratified Squamous Epithelium, Stratum Corneum, Stratum Granulosum

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Immunological defense: skin is not vascularized when cut yourself and bleed is when go through plasma membrane, fat cells in hypodermis. Describing epithelium: simple or stratified, describe shape of top cell layer, ciliated or not, epithelia. Fuchsin sulphurous acid: fuchsin sulphurous acid (f[so4h]2): colorless leukofuchsin, aka schiff reagent, shift-base reaction, stain bm, cross-link molecules and give purple color, basement membrane is always there, but need special stain or else will not see it. P. a. s reaction: ethanol dissolves fat in fixation process, so hypodermis is not visible, white ghost cells. Composition of basement membrane: proteoglycans, glycoproteins, collagen, a layer of reticular fibers underlies bm. Typical proteoglycan: protein core from which spikes stick out, example: heparan sulfate. Composition: 50% sugars & 50% protein, function: gags are negatively charged and hold water, retain water, give resistance / sponginess. Glycoprotein: 10% sugars and 90% protein, end link sugar attached to protein backbone, examples: lamini, entactin, nidogen, fibronectin.

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