BIOL 202 Lecture Notes - Lecture 13: Du145, Pc3, Neomycin
31 May 2016
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Locating genes (or phenotypes) before the human genome was sequenced: the power of somatic cell hybrids. Exam: 12 from hipfner and 28 from dankort. 23 human chromosome pairs: 22 autosomal (1-22 based on length), 1 sex. M = stands for minute messed or. Using cell fusion, chromosomes can be transferred from one cell to another: can fuse myeloma cell to a plasma cell to make an immortalized cell line. One of those two cell lines is metastatic and one isn"t. In cancer genomes, you can lose whole chromosomes. Rna-based virus particle binds to two cells (like human to rodent cell: has a lipid bilayer on the outside; so lipid-lipid interactions. A single cell with two nuclei is formed. At the first mitotic division, the human and rodent chromosomes attach to a single spindle apparatus. Requirement for using somatic cell hybridization to identify gene location.
