BIOL 202 Lecture Notes - Lecture 23: Alcohol Tolerance, Zygosity, Bk Channel
5 Nov 2015
School
Department
Course
Professor
Document Summary
Transgenes can do many things: label cells and tissues, tag proteins, label subcellular structures, cause gain- or loss-of-function phenotypes, act as indicators of cellular events. Transcriptional reporter: most regulatory elements are located in upstream region of gene of interest, copy upstream sequence and put it in front of gfp, can activate gfp using regulatory elements and inject into an organism. Combinatorial expression: can express multiple fluorescent proteins, three constructs expressed in a mouse"s brain, different dosage of each colour in each neuron, mice can express different combinations of the construct. Inject dna into nucleus of a fertilized egg - it integrates into chromosome. Implant injected fertilized eggs into surrogate mother to develop o o: multiple copies of transgene will randomly integrate into genome of some cells (occasionally future germ cells, breed and genotype offspring to identify fully transgenic animals, knockout mice. Balancer chromosome - will kill embryo if you have 2 copies of it.
