BIOL 202 Lecture Notes - Lecture 16: In Situ Hybridization, Transposable Element, P Element
Document Summary
Gene chips containing spots of cdnas or oligonucleotide probes for each gene in the genome they are hybridized with fluorescently-labelled cdnas from tissues of interest to see what genes are expressed. Microarrays can be used to type tumors: similarities and differences in gene expression between different patient tumor samples can be used to classify tumors according to origin and mechanism. Run 2dge to separate by charge, then size. Cut out protein spots from gel and sequence to see what proteins are present in a tissue. Hybridization to tissue sections or whole organisms to detect cells making mrna of gene of interest. Rna probe is used that is complementary to mrna (antimrna) Probe is radioactively labeled or labeled with a tage for colourimetric enzyme detection. Promoter of gene of interest is fused to a reporter such as gfp. Expression of reporter gene represents endogenous gene expression. Immunocytochemistry the use of ab to detect specific proteins in tissue sections or whole organisms.
