BIOL 200 Lecture Notes - Lecture 25: Nikel, Zinc Finger, Xeroderma Pigmentosum
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It must be rendered single-stranded prior to use it will act as a complementary probe. Pcr can also be used to make fluorescently labelled dna probes (cid:120) Dna (cid:120) the non-labelled probe can be used in hybridizations just as a radioactively-labelled dna probe (cid:120) detection requires a second hybridization step that includes antibodies or fluorescently-labelled conjugates. Nucleic acid hybridization techniques enable dna and rna detection (cid:120) nucleic acids of complementary sequences can base pair to form double-stranded hybrids. Analysis of nucleic acids by transferring to solid state support (nylon, nitrocellulose) (cid:120) both dna and rna can be separated according to size using an agarose gel. Once covalently bound, the levels and positions are permanently recorded (cid:120) the nucleic acids are then bound covalently (cid:120) permanent record of abundance and the size of the molecules following separation (cid:120) the (cid:862)(cid:271)lot(cid:863) (cid:272)a(cid:374) (cid:271)e (cid:862)h(cid:455)(cid:271)(cid:396)idized(cid:863) (cid:449)ith p(cid:396)o(cid:271)es. Pcr can also be used for genotyping (cid:120) an example: a specific allele of the breast.
