BIOL 200 Lecture Notes - Lecture 14: Jigsaw Puzzle, Dna Sequencing, Prokaryote
Document Summary
Genomics: determining the dna sequence of the genome, identifying the location of genes, commonly referred to as annotation. Annotation -> identifying various features within the sequence. Since the epstein-barr virus was sequenced in 1984, the dna sequence from thousands of viral, organellar, prokaryotic and eukaryotic genomes have been determined: ex. Determined the sequence of various model organisms: yeast, roundworm, fruit y, zebra sh, mice, plant. There are >2000 complete/ongoing eukaryotic genome projects: chimpanzee, corn, puffer sh (small vertebrate genomes, mosquito, rice. Take advantage of sequence overlap: blue letters in diagram represent overlap in sequence between 1 and 2 to come up with a consensus sequence/reconstruction. Put fragments together by taking advantage of overlap -> stack fragments on top of another with an offset -> tiling paths. Regions of tiling paths = contiguous sequences or contigs. Scaffold contains contigs and gaps (no sequence info) Dideoxy dna sequencing is no longer used to sequence genomes: replaced by next generation sequencing:
