BIOL 200 Lecture Notes - Lecture 21: Ethidium Bromide, Southern Blot, Acrylamide
7 Jun 2016
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Document Summary
Separation of dna fragments by gel electrophoresis: connection with southern blot, apply electric field across length of gel, uses agarose or acrylamide gel. Interferer with migration of molecules: larger fragments run slower, smaller fragments run faster, visualization using fluorescent dna binding dye ethidium bromide. Shine uv light to view: moves negative positive. Dna has negative charge will run towards positive end: dna in added in small wells at top of gel. Amplification of specific dna fragments by polymerase chain reaction (pcr: pcr amplification = in vitro dna replication of a specific dna fragment through multiple replication cycles in which the amount of dna doubles in each cycle. Twice as much at end as beginning: ex. After 35 cycles the amount of the amplified fragment would be increased. Can be done in an afternoon timewise: ultimate sensitive. Can detect a single molecule in a tube.
