BIOL 200 Lecture Notes - High Fidelity, Hydroxy Group, Agarose Gel Electrophoresis
6 Apr 2012
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Document Summary
Dna cloning, polymerase chain reaction (pcr) and dna sequencing are the fundamental techniques used in molecular biology. Other, more complex techniques borrow general aspects from these 3 techniques. For many reactions involving dna in molecular biology, a large amount of dna is required, which can be difficult to obtain without the use of mainly dna cloning and. Plasmids are circular, double stranded dna molecules (dsdna) which are much smaller than genes (usually 2000-10000 kbp) Plasmids are the most common vectors used in dna technology: vectors are media which carry specific genes of interest. They are extrachromosomal, meaning they are not part of the genome. They can be found in bacteria (mainly prokaryotes) and lower eukaryotes. The replication of plasmids occurs before cell division. The e. coli plasmid is a common vector used in labs. The polylinker contains multiple endonuclease restriction sites which scientists can cleave and genes of interest can be inserted.
