BIOL 200 Lecture Notes - Phosphodiester Bond, Intron, Transcription Factor Ii D
Document Summary
Rna polymerase grabs 1st rntp (complementary base pairs w/ 1st dna base) Transcription starts in 3" direction of template, synthesis of rna is 5" to 3" transcription begins at nucleotide +1. Promoter (signal for rna to know general area of start site) Coding sequence template strand (3" to 5") complements with rna phosphodiester bond formed, rna polymerase goes from initiation to elongation. Can have multiple transcription bubbles (rna polymerases can transcribe simultaneously at diff. Prokaryoties operons: multiple genes stitched together few non-coding gaps in dna. Eukaryotes genes are on different chromosomes but can be shuttled to the same location. 5" end: enzymes add the 5" cap (5" 5" linkage) prevents degradation splicing of introns done by enzymes independent of polymerase if enzymes that degrade dna are around, the poly a tail protects the 3" end. Utr: parts of genes before start site and after stop site. Protect against degradation, may have other functions splicing can be cell specific (diff.
