BIOL 200 Lecture Notes - Lecture 12: Sulfate Adenylyltransferase, Apyrase, Pyrosequencing
23 Jan 2018
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October 13th: gattaca: genomics and next generation dna sequencing. Omics era: the study of the whole genome, determining the dna sequence of the genome. Where the genes are: annotation (mapping of gene loci) What genes do: genomics, functional genomics, proteomics, evolutionary genomics, transcriptomics, phenomics, spliceomics, metabolics: Influenza: yersinia pestis, chimpanzee, maize, pufferfish, tunic-h genome, mosquito, banana, rice, etc, fragment genome through mechanical shearing, assembly: assume that overlapping sequences are part of the same genes (contigs: contiguous sequences) More of these than chromosomes because you don"t necessarily know where the. Usually rich in transposons and repetitive elements and you don"t know which to put where: method is prone to having gaps scaffolds go. Massively parallel: move through many at the same time. Microfluidics: do not require a large volume of solution. Fixed synthesis: either on a bead or on a small platform. Good with small read, very good with large reads: general sequencing pipeline: Amplification like in pcr (to increase signal)
